
- Is Sam really dead?
- How many people has Ava killed since she was at Cold Oaks?
- Did anyone survive the Roadhouse fire?

- It was Ava controlling the demons.
- Ava has been at Cold Oaks for five months.
- The YED put them all here to battle for Top Dog.
- Whoever survives will lead Hell's Army.

- At the start of the episode, Dean is waiting in the car for Sam when the Yellow-Eyed Demon comes and gets Sam. The radio starts to flutter in and out which in countless past episodes has been a sign that there is a supernatural presence close. Why would he tap on the radio trying fix it?
- At the very start, when Sam wakes up in the ghost town, there is a visible church--wouldn't it be sensible to automatically seek refuge there?
- When Dean is talking to Ash on the Roadhouse telephone, Ash is wearing his watch on his left wrist, yet after the Roadhouse fire when Dean finds Ash's body, his watch has changed wrists and is now on his right wrist.

- Foreplay/Long Time - Boston

- Signature: D. HasselhoffReferences David Hasselhoff, whose most notable roles are Michael Knight on Knight Rider from 1982-86 and Lt. Mitch Buchannon on Baywatch from 1989-2000.
- Sam: It's a brave new world.Referring to Aldus Huxley's 1932 novel, Brave New World, that describes an ironic utopia achieved through reproductive technology and biological engineering. Huxley in turn borrowed the line from Shakespeare's The Tempest.

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Driven to the edge of emotional endurance, Dean makes a sacrifice that leads to a cosmic showdown with the yellow-eyed demon... and to consequences even more far-reaching.